This project is funded by Norwegian Financial Mechanism 2014-2021, under Grant Agreement no. 27/2020

Programme: Research RO-02 operated by UEFISCDI

Funding: 1.147.010,00 euro

Timeframe: 2020-2023 

Granular activated algae technology for wastewater treatment and resources recovery

Code: RO-NO-2019-0691

Acronym: GRAALrecovery



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Journal of Science and Arts, 2021, Vol. 57(4), pages 1111-1120


UV-VIS analysis of granular activated algae chlorophyll content


Marius Bumbac, Cristina Mihaela Nicolescu, Radu Lucian Olteanu, Olga Tiron, Elena Elisabeta Manea, Costel Bumbac, Laura Monica Gorghiu, Cristiana Radulescu, Geanina Stanescu, Bogdan Catalin Serban, Octavian Buiu


Abstract. Chlorophyll-a is the pigment whose concentration is an important indicator for the development of microalgae biomass. This study aims at evaluating the concentration of chlorophylls in the biomass of granular activated algae, using acetone and 90% ethanol for the extraction procedures applied. Concentrations of chlorophyll a, b and c were determined by applying three calculation types: methods proposed by Jeffrey and Humphrey for the extracts in acetone, Ritchie method for ethanolic extracts, and monochromatic method with acidification for ethanolic extracts. Experimental findings show that the solvent 90% ethanol is more efficient than acetone for extracting chlorophyll from biomass of granular activated algae. Also, by comparing results obtained by the two methods proposed for ethanolic extracts, it was found that the values obtained through the acidification method are influenced by the volume of hydrochloric acid added to the organic chlorophylls extract.

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